CHOPCHOP

Target specific region of gene:

Coding region

All exons (inc. UTRs)

Splice sites

5' UTR

3' UTR

Promoter

Only target exon(s):

Restrict targeting:

Search exons and immediate short flanking regions.

Only search within the exon.

Restriction enzymes:

Company preference:

Minimum size of restriction enzyme binding site:

sgRNA length without PAM:

5'-PAM:

TTN

TTTN

Custom PAM:

Self-complementarity (Thyme et al.):

Check for self-complementarity

Check for complementarity versus backbone:

Standard backbone

Extended backbone

Custom backbone:

Distance between TALENs:

Size of TALENs:

Number of mismatches to search for when finding off-targets:

G repeat variable diresidue (RVD):

Design primers

Product size:

From: to:

Primer size:

From: to: Optimal:

Primer Tm:

From: to: Optimal:

Minimum distance from primer to target site: