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Target
RefSeq/ENSEMBL/gene ID or genomic coordinates.
Target
Paste fasta entry here.
In
Homo sapiens (hg38/GRCh38) GENCODEv29
Mus musculus (mm10/GRCm38) GENCODEvM19
In
Aedes aegypti (AaegL3)
Aedes aegypti (AaegL5)
Aedes albopictus (GCA_001876365.2)
Acyrthosiphon pisum (Acyr_2.0)
Anopheles funestus (AfunF1)
Anopheles gambiae (anoGam1)
Anopheles gambiae (AgamP4)
Anopheles stephensi (GCA_013141755.1)
Anopheles stephensi (Indian, AsteI2)
Anopheles stephensi (SDA-500, AsteS1)
Anopheles albimanus (AalbS2)
Apis mellifera (HAv3.1)
Apolygus lucorum (GCA_009739505.2)
Archegozetes longisetosus (GCA_018873245.2)
Bactrocera cucurbitae (ASM80634v1)
Bactrocera dorsalis (ASM78921v2)
Bactrocera oleae (GCA_001188975.2)
Bemisia tabaci (ASM185493v1)
Bombyx mori (release 31)
Camponotus floridanus (GCF_003227725.1)
Ceratitis capitata (Ccap1.0)
Ceratitis capitata (Ccap_2.1)
Clitarchus hookeri (GCA_002778355.1)
Cimex lectularius (GCF_000648675.2)
Culex quinquefasciatus (CpipJ2)
Dalotia coriaria (Dcor_v1)
Diaphorina Citri (GCF_000475195.1)
Drosophila melanogaster (dm3)
Drosophila melanogaster (dm6)
Drosophila suzukii (Dsuz 1.0)
Grape Phylloxera (Daktulosphaira vitifoliae) (v3.1)
Haemaphysalis longicornis (GCA_013339765.2)
Harpegnathos saltator (GCF_003227715.1)
Hermetia illucens (iHerIll2.2.curated.20191125)
Mayetiola destructor (v1.0)
Ixodes scapularis (IscaW1)
Ixodes scapularis (GCF_016920785.2)
Lepeophtheirus salmonis oncorhynchi (UVic_Lsal_1.2)
Lepeophtheirus salmonis salmonis (LSalAtl2S)
Lasius niger (GCA_001045655.1)
Lucilia cuprina (GCA_022045245.1)
Lutzomyia longipalpis (LlonJ1.5)
Manduca sexta (Mansex1.0)
Nilaparvata lugens (GCF_014356525.2)
N. vitripennis (Nvit1.0)
Nasonia vitripennis (Nvit2.1)
Papilio polytes (Ppolytes v1)
Phlebotomus papatasi (Ppapl1.5)
Pieris brassicae (GCF_905147105.1)
Rhodnius prolixus (RproC3)
Rhynchophorus ferrugineus (ASM1446268v1)
Schistocerca americana (GCA_021461395.2)
Tetranychus urticae (Sept 2009)
Thrips palmi (GCA_012932325.1)
Tribolium castaneum (Tcas5)
Amphilophus citrinellus (ASM1343575v1)
Astatotilapia burtoni (AstBur1.0)
Astyanax mexicanus (AstMex102)
Astyanax mexicanus (surface) (ensembl v2)
Austrofundulus limnaeus (GCF_001266775.1)
Betta splendens (v1.1)
Betta splendens (fBetSpl5.2)
Betta splendens (BetSpl5)
Bos taurus (Btau 5.0)
Bos taurus (bosTau9)
Bos taurus (ARS-UCD1.2.108)
Bos taurus indicus (GCF_000247795.1)
Bubalus bubalis (GCF_003121395.1)
Chinese Hamster Ovary CHO-K1 (GCF_003668045.3_CriGri-PICRH-1.0)
Canis familiaris (CanFam3)
Canis familiaris (CanFam4)
Ciona intestinalis (KH2012)
Callithrix jacchus (C_jacchus3.2.1)
Capra hircus (GCF_001704415.1)
Carcharodon carcharias (GCA_003604245.1)
Chlorocebus sabaeus - Vero cells (GCA_015252025.1)
Chrysemys picta bellii (GCA_000241765.2)
Coturnix japonica (GCF_001577835.1)
Cyprinodon variegatus (GCF_000732505.1)
Danio rerio (danRer10/GRCz10)
Danio rerio (danRer11/GRCz11)
Equus caballus (EquCab3)
Felis Catus (felCat9)
Gadus morhua (GCA_902167405.1)
Gasterosteus aculeatus (gasAcu1)
Gallus gallus (galGal4)
Gallus gallus (galGal5)
Gallus gallus (galGal6)
Hippoglossus hippoglossus (fHipHip1.pri)
Homo sapiens CHM13 T2T (v1.1)
Homo sapiens (hg19/GRCh37)
Homo sapiens (hg38/GRCh38)
Ictalurus punctatus (GCF_001660625.1)
Ictalurus punctatus (GCF_001660625.2)
Ictalurus punctatus (v3/GCA_001660625.3)
Lates calcarifer (TLL_Latcal_v3)
Larimichthys crocea (GCA_900246015.1)
Labeo rohita (GCA_022985175.1)
Larimichthys crocea (GCA_000972845.2)
Lepisosteus oculatus (LepOcu1)
Leucoraja erinacea (GCF_028641065.1)
Macaca Nemestrina (GCA_000956065.1)
Mesocricetus auratus - BHK 21 cells (GCF_017639785.1)
Maylandia zebra (M_zebra_UMD2a/GCA_000238955.5)
Microtus Ochrogaster (GCF_000317375.1_MicOch1.0)
Mus musculus (mm10/GRCm38)
Mus musculus (GRCm39)
Mustela putorius furo (MusPutFur1.0/musFur1)
Macaca mulatta (Mmul_10)
Nothobranchius furzeri (NotFur1/Stanford)
Nothobranchius furzeri (Nfu_20140520/Jena)
Oikopleura dioica (Odioica)
Ompok bimaculatus (GCA_009108245.1)
Oncorhynchus kisutch (GCA_002021735.2/Okis_V2)
Ooceraea biroi (GCA_003672135.1)
Oryctolagus cuniculus (GCA_000003625.1)
Oryzias latipes (oryLat2)
Oryzias latipes (ASM223467v1)
Oncorhynchus mykiss (Omyk_1.0)
Oreochromis niloticus (ASM185804v2)
Ovis aries (Oar_v3.1)
Peromyscus californicus (GCA_007827085.3)
Rattus norvegicus (rn6)
Rattus norvegicus (mRatBN7.2)
Salmo salar (GCF_000233375.1)
Salmo salar (Ssal_v3.1)
Salvelinus namaycush (GCF_016432855.1)
Sus scrofa (Sscrofa11.1)
Sparus aurata (GCA_900880675.1)
Xenopus laevis (Xenla9.1/JGI v9.1)
Xiphophorus variatus (xvar_V1.0)
Xenopus laevis (v9.2)
Xenopus_laevis (v10.1)
Xenopus tropicalis (UCB_Xtro_10.0)
Exaiptasia pallida isolate CC7 (GCA_001417965.1)
Nematostella vectensis (nemVec1)
Nematostella vectensis (jaNemVect1.1)
Mnemiopsis leidyi (GCA_000226015.1)
Methanococcus maripaludis JJ (DSM 2067) (CP026606.1)
Methanosarcina acetivorans C2A (NC_003552.1)
Cladocopium goreaui (SymbC1)
Lytechinus pictus (GCA_015342785.1)
Lytechinus variegatus (Lvar_2.2)
Patiria miniata (ASM1570657v1)
Strongylocentrotus purpuratus (EchinoBase v3.1)
Biomphalaria glabrata (BglaB1)
Octopus bimaculoides (PRJNA270931)
Ancylostoma ceylanicum (PRJNA231479)
Caenorhabditis elegans (ce11/UCSC)
Nippostrongylus brasiliensis (GCA_900618405.1)
Pristionchus pacificus (TAU2011)
Acidovorax citrulli str. M6 (CP029373.1)
Acinetobacter baumannii (NZ_CP015121.1)
Acinetobacter baumannii ATCC 19606 (ASM1139851v1)
Actinoplanes deccanensis YP-1
Anabaena (PCC 7120)
Azotobacter vinelandii DJ (NC_012560.1)
Bdellovibrio bacteriovorus (ASM19617v1)
Bacteroides fragilis 638R (FQ312004.1)
Bacteroides fragilis 9343 (NC_003228.3)
Bacillus RZ2MS9 (MJBF01)
Bacillus RZ2MS9 (CP049978.1)
Bacillus cereus ATCC14579 (NC_004722.1)
Bacillus licheniformis ATCC 14580 (GCF_000011645.1)
Bacillus megaterium (ATCC 14581) (CP023317.1)
Bacillus subtilis 168 (NC_000964.3)
Bacteroides thetaiotaomicron (FR901300.1)
Bifidobacterium longum (GCA_000196555)
Burkholderia thailandensis E264 (GCA_003568605.1)
Bacillus thuringiensis serovar israelensis str. AM65-52 (GCF_003445395.2)
Bordetella pertussis B1917 (GCF_000193595.2)
Candidatus Carsonella ruddii (NC_018417.1)
Caulobacter crescentus (NA1000)
Chlamydia psittaci (GCA_000204255.1)
Chryseobacterium gleum ATCC35910 (GL379781.1)
Clostridium Butyricum JKT37 (GCA_003849835.1)
Clostridium acetobutylicum ATCC 824 (GCF_000008765.1)
Clostridium beijerinckii 6423 (GCF_900010805.1)
Clostridium botulinum ATCC 19397 (NC_009697.1)
Corynebacterium glutamicum ATCC 13032 (GCF_000011325.1)
Corynebacterium pseudotuberculosis 1002 (GCF_000144935.1)
Coxiella burnetii RSA 493 (GCF_000007765.2)
Cupriavidus malaysiensis USMAA1020 chr1 (CP017754.1)
Cupriavidus malaysiensis USMAA1020 chr2 (CP017754.1)
Cyanobacterium aponinum PCC 10605 (CP003947.1)
Campylobacter jejuni NCTC11168 (GCF_000009085.1)
Dermacoccus abyssi str. HZAU 226 (CP043031.1)
Escherichia coli O127:H6 str. E2348/69 (GCA_000026545.1)
Escherichia coli (str. CFT073/ASM744v1)
Escherichia coli str. BL21 (DE3) (NC_012971.2)
Escherichia coli (str. K-12/MG1655)
Escherichia coli (Nissle 1917/CP007799.1)
Escherichia coli (C321.ΔA/GCA_000474035.1)
Escherichia coli BW25113 (NZ_CP009273.1)
Enterococcus faecalis OG1RF (GCA_000172575.2)
Enterococcus faecalis VPE25 (LT615366.1)
Eubacterium limosum ATCC 8486 (NZ_CP019962.1)
Francisella tularensis sp. holarctica FSC200 (GCA_000168775.2)
Hyphomicrobium denitrificans ATCC 51888 (GCF_000143145.1)
Klebsiella pneumoniae str. SGH10 (CP025080)
Lactobacillus delbrueckii (ATCC 11842)
Lachnoclostridium phytofermentans (NC_010001.1)
Lactobacillus casei (GCA_000829055.1)
Lactobacillus plantarum WCFS1 (firmicutes) (GCF_000203855.3)
Legionella pneumophila (GCF_000008485.1)
Leptospira biflexa (GCF_000017685.1)
Leptospira interrogans serovar Copenhageni (GCF_002073495.2)
Leptospira interrogans serovar Copenhageni str. M20 (GCA_000332535.2)
Listeria monocytogenes EGD-e (GCF_000196035.1)
Lysobacter enzymogenes (NZ_CP013140.1)
Methylococcus capsulatus Bath (GCF_000008325.1)
Mycobacterium avium subsp. paratuberculosis strain K-10 (GCA_025665475)
Mycobacterium avium subsp. paratuberculosis strain MAP4 (GCA_000390085)
Mycobacterium bovis AF2122/97 (NC_002945.4)
Mycobacterium liflandii (GCA_000026445.2)
Mycobacterium marinum M (ASM1834v1)
Mycobacterium neworleansense ATCC 49404 (GCF_001245615.1)
Mycobacterium ulcerans (GCA_000013925.2)
Mycobacteroides abscessus ATCC 19977(MAb) (GCA_000069185.1)
Mycobacterium smegmatis MC2 155 (GCF_000015005.1)
Mycolicibacterium neoaurum NRRL B-3805 (GCA_001580405.1)
Mycoplasma genitalium (NC_000908.2)
Mycoplasmoides gallisepticum str. R(low) (NC_004829.2)
Neisseria gonorrhoeae FA 1090 (NC_002946.2)
Nocardia terpenica strain NC_YFY_NT001 (NZ_CP023778.1)
Nostoc punctiforme PCC 73102 (GCF_000020025.1)
Mycobacterium tuberculosis H37Rv (ASM19595v2)
Orientia tsutsugamushi (GCF_900327275.1)
Paenibacillus thiaminolyticus (GCF_002161855.1)
Pantoea agglomerans (33.1)
Pantoea sp. PNA 14-12 (GCA_004364735.1)
Pectobacterium aroidearum L6 (GCF_015689195.1)
Pectobacterium brasiliense 1692 (GCF_009873295.1)
Pectobacterium carotovorum subsp. carotovorum PC1 (GCF_000023605.1)
Pseudomonas aeruginosa (UCBPP-PA14)
Pseudomonas aeruginosa PAO1 (GCF_000006765.1)
Pseudomonas stutzeri A1501 (GCF_000013785.1)
Pseudomonas syringae pv. tomato str. DC3000 (NC_004578.1)
Pantoea agglomerans (NZ_CP014129.1)
Pseudomonas cichorii JBC1 (PRJNA232591)
Phocaeicola dorei str. DSM 17855 (GCA_013009555.1)
Rhizobium etli (NC_007761.1)
Rhodobacter capsulatus SB 1003 (CP001312.1)
Rhodobacter sphaeroides 2.4.1 (NZ_CP030271.1 (chr1))
Rhodobacter sphaeroides 2.4.1 (NZ_CP030272.1 (chr2))
Rhodobacter sphaeroides (KD131)
Rhodopseudomonas palustris CGA009 (NC_005296.1)
Salmonella enterica subsp. (GCF_000022165.1)
Salmonella enterica subsp. Typhimurium str. LT2 (GCF_000006945.2)
Serratia marcescens str. S5 (ASM801186v1)
Shewanella oneidensis MR-1 (AE014299.2)
Shigella flexneri 1c (CP020753)
Shigella flexneri 5a M90T (NZ_CP037923.1)
Staphylococcus aureus (MSSA-GP01, v1.0)
Staphylococcus aureus (MRSA-ATCC 43300, v1.0)
Staphylococcus aureus subsp. aureus USA300_FPR3757 (GCF_000013425.1)
Streptococcus mutans (GCA_000007465.2)
Streptomyces ambofaciens (GCF_001267885.1)
Streptomyces virginiae NRRL ISP-5094 (GCA_000720455.1)
Streptomyces coelicolor M145 (ASM20383v1)
Streptomyces platensis (DSM-40041)
Streptococcus pyogenes M1 GAS (GCA_000321355.1)
Synechococcus sp. PCC 7002 (CP000951.1)
Teredinibacter turnerae str. T7901 (NC_012997.1/GCF_000023025.1)
Thalassomonas viridans (GCF_000948985.1)
Thermoanaerobacter ethanolicus JW 200 (GCF_003722315.1)
Treponema caldarium (GCF_000219725.1)
Vibrio natriegens (NZ_CP009977.1)
Xenorhabdus nematophila (GCF_000252955.1)
Aspergillus fumigatus (Af293)
Aspergillus niger (NRRL3)
Aspergillus oryzae (RIB40)
Aspergillus pseudoterreus ATCC 32359 (v1.0)
Arthrobotrys oligospora (GCF_000225545.1)
Aspergillus niger (ATCC 1015)
Botrytis cinerea (ASM83294v1)
Candida albicans (A22)
Candida auris (GCA_002759435.2)
Candida glabrata (CBS138)
Candida haemulonii (GCA_002926055.1)
Candida krusei (GCA_003054445.1)
Candida lusitaniae (GCA_000003835.1)
Candida parapsilosis (GCA_000182765.2)
Candida tropicalis (MYA-3404)
Collectotrichum graminicola M1.001 (Colgr1)
Colletotrichum higginsianum IMI 349063 (GCF_001672515.1)
Cochliobolus heterostrophus C5 (v2.0)
Drechslerella dactyloides (GCA_028984885.1)
Fusarium fujikuroi IMI58289 (GCA_900079805.1)
Fusarium graminearum (GCF_000240135.3)
Grifola frondosa (GCA_001683735.1)
Histoplasma ohiense (GCA_017607445.1)
Kluyveromyces lactis (ASM251v1)
Lipomyces starkeyi (GCA_001661325.1)
Metarhizium anisopliae (MAN 1.0)
Monilinia fructicola str. Mfrc123 (GCA_008692225.1)
Mortierella alpine ATCC 32222 (GCA_000240685.2)
Neurospora crassa (GCA_000182925.2)
Ogataea polymorpha NCYC495 leu1.1 (Hanpo2)
Paecilomyces variotii (CBS101075)
Penicillium roqueforti (FM164)
Pestalotiopsis sp. IQ-011 (Pestalotiopsis_sp_IQ-011)
Pochonia chlamydosporia (GCF_001653235.2)
Puccinia triticina (GCA_000151525.1)
Purpureocillium lilacinum (GCA_001653205.1)
Pichia pastoris (PRJEA62483)
Rasamsonia emersonii (GCA_000968595.1)
Rhodosporidium toruloides IFO0880 (GCA_000988875.2)
Saccharomyces cerevisiae (sacCer3/S288c)
Saccharomyces cerevisiae str. CEN.PK113-7D (GCA_002571405.2)
Schizosaccharomyces pombe (ASM294v2.30)
Sporisorium scitamineum 39 (GCA_001010845.1)
Starmerella bombicola (JCM_9596_assembly_v001)
Thecaphora thlaspeos (GCA_900291925.1)
Trichoderma brevicrassum TC967
Trichoderma harzianum T22 (TriharT22)
Trichoderma reesei (GCA_000167675.2)
Trichophyton interdigitale MR816 (GCA_000622975)
Trichophyton rubrum CBS 118892 (GCA_000151425)
Ustilago maydis (GCA_000328475.2)
Yarrowia lipolytica (GCF_000002525.2)
Yarrowia lipolytica (W29)
Zygosaccharomyces rouxii (ASM2636v1)
Zymoseptoria tritici (GCA_000219625.1)
Leishmania braziliensis (MHOM/BR/75/M2904)
Leishmania major Friedlin (LmjF.01)
Leishmania major Friedlin (TritrypDB 46)
Neospora caninum Liverpool 2019 (ToxoDB-58_Ncaninum_Liverpool_2019)
Plasmodium berghei (ANKA v2.0)
Plasmodium cynomolgi (GCF_000321355.1)
Plasmodium falciparum (3D7 v3.0)
Opisthorchis viverrini (GCF_000715545.1)
Schistosoma haematobium (GCF_000699445.2)
Schistosoma mansoni (Smansoni_v7)
Schistosoma mansoni (SMA_v10)
Theileria parva Muguga (2021-01-12)
Trypanosoma cruzi (TcruziSylvioX10-1-2012)
Trypanosoma brucei (TREU927)
Toxoplasma gondii GT1 (ToxoDB-28)
Toxoplasma gondii ME49 (ToxoDB-28)
Actinidia chinensis (v3.0)
Aquilegia coerulea (v3.1)
Arachis hypogaea (GCA_003086295.2)
Amaranthus hypochondriacus (v2.1)
Arachis duranensis (Aradu 1.0)
Arachis ipaensis (Araip 1.0)
Arabidopsis thaliana (Araport11)
Arabidopsis thaliana (Araport11)
Brassica rapa R500 (NC_024804.2)
Capsella rubella (v1.0)
Camelina sativa (v2)
Cannabis sativa (GCA_900626175.2)
Capsicum annuum (GCA_000710875.1)
Ceratopteris richardii (GCA_020310875.1)
Cardamine hirsuta (v1.0)
Cephalotus follicularis (v3.0)
Chrysanthemum seticuspe (CSE_r1.0)
Chlorella sorokiniana (CSI2_1230)
Cicer arietinum (ASM33114v1)
Coffea canephora (v1.0)
Cucumis melo (Melon DHL92 v4.0)
Cucumis sativus (GCF_000004075.3)
Dendrobium catenatum (ASM160598v2)
Eucalyptus grandis (v2.0)
Emiliania huxleyi (CCMP1516)
Eutrema salsugineum (v1.0)
Eragrostis tef (1.1.2)
Fragaria x ananassa (FaRR1)
Glycine max (GCA_000004515.3)
Gossypium hirsutum (HAU_v1.1)
Hordeum vulgare, morex cultivar (Morex V2)
Ipomoea trifida (NSP306)
Ipomoea triloba (NSP323)
Lupinus angustifolius (GCF_001865875.1)
Marchantia polymorpha (MpTak1v5.1)
Nannochloropsis oceanica (IMET1.v2)
Nymphaea thermarum (ASM1179976v1)
Malus domestica (GCF_002114115.1)
Oryza sativa (MSU-version_7.0)
Oryza sativa (Kitaake v3.1)
Petunia axillaris (v1.6.2)
Petunia hybrida (R27v011)
Panicum virgatum (Panicum virgatum-v4)
Physcomitrella patens (Ppatens_318_v3)
Phalaenopsis equestris (ASM126359v1)
Populus deltoides (WV94 v2.1)
Populus trichocarpa (v3.1)
Salix purpurea (v5.1)
Schrenkiella parvula (v2.2)
Sesamum indicum (GCF_000512975.1)
Solanum lycopersicum (SL3.0)
Solanum lycopersicum (SL3.1)
Sorghum bicolor (Phytozome v3.1.1)
Spirodela polyrhiza (v2)
Utricularia gibba (v1.1F)
Thlaspi Arvense (Genome Version 2.0 MN106/GCA_911865555.2)
Triticum aestivum (IWGSC v1.1) Chr 1 & 2 only!
Vitis vinifera (12X)
Zea mays (B73 AGPv4)
African Swine Fever Virus Kenya (IX 1033)
Autographa californica nucleopolyhedrovirus (AcMNPV) (NC_001623.1)
Bovine diarrhea virus (NC_001461.1)
Bovine herpesvirus 1 (NC_001847.1)
Enterococcus faecalis phage VPE25 (GCF_000007785.1)
Enterobacteria phage lambda (J02459.1)
Gallid herpesvirus 1 (NC_006623.1)
Gallid herpesvirus 2 (str. CVI988, DQ530348.1)
Gallid herpesvirus 2 (str. RB-1B) (EF523390.1)
Gallid herpesvirus 3 (str. SB-1, HQ840738.1)
Hepatitis B (AB602818.1)
Hepatitis C (M58335.1)
Human herpesvirus 1 (str. 17, JN555585.1)
Human herpesvirus 2 (str. 333)
Human herpesvirus 2 (str. HG52)
Human herpesvirus 2 (str. SD90e)
Human herpesvirus 4 (str. Akata)
Human herpesvirus 6A (U1102_X83413)
Human herpesvirus 6B (Z29_NC000898)
Human herpesvirus 7 (AF037218.1)
Human T-cell lymphotropic virus type I (J02029.1)
HIV-1 (AF033819.3)
Meleagrid herpesvirus 1 strain FC126 (AF291866.1)
Monkeypox virus Zaire -96-I-16 (NC_003310)
Myxoma Virus (GCA_000843685.1)
Rotavirus A (GCA_000880735.1)
Salmonella phage SSE-121 (GCF_001041715.1)
Simian foamy virus isolate BAK74 (JQ867464)
Vaccinia virus (str. WR/AY243312.1)
Wuhan Coronavirus (nCORV-2019)
Acanthamoeba castellanii (GCF_000313135.1)
Amphimedon queenslandica (GCF_000090795.1)
Aurantiochytrium limacinum ATCC MYA-1381 (Aurli1)
Aphanomyces euteiches str. ATCC 201684 (GCA_021527685)
Babesia bovis T2Bo (BbovisT2Bo)
Chlamydomonas reinhardtii (v5.6)
Corallochytrium limacisporum (Hawaii) (v01)
Cryptosporidium parvum Iowa II (GCF_000165345.1)
Ectocarpus (EctsiV2)
Microchloropsis gaditana (GCA_000240725.1)
Naegleria gruberi (GCF_000004985.1)
Ostreococcus tauri RCC4221 (v3.0)
Phaeodactylum tricornutum (ASM15095v2)
Pseudonitzschia multistriata (PsnmuV1.4)
Schmidtea mediterranea (schMed4)
Tetrahymena thermophila (MAC)
Tetrahymena thermophila (MIC)
Add
new species.
Using
CRISPR/Cas9
CRISPR/Cas9 nickase
CRISPR/Cpf1 or CasX
CRISPR/Cas13 (eg. C2C2)
TALEN
Change default PAM and guide length in Options.
For
knock-out
knock-down
knock-in
activation
repression
nanopore enrichment
Presets can be adjusted in Options.
General
Cas9
Cpf1
Cas13
Cas9 nickase
TALEN
Primers
Target specific region of gene:
Coding region
All exons (inc. UTRs)
Splice sites
5' UTR
3' UTR
Promoter
Only target exon(s):
Restrict targeting:
Search exons and immediate short flanking regions.
Only search within the exon.
Isoform consensus determined by:
Intersection (only searches regions present in all isoforms)
Union (searches all exons in all isoforms)
Pre-filtering:
Minimum required GC [%] content has to be between min:
and max:
Self-complementarity has to be below:
Restriction enzymes:
Company preference:
Life Technologies
Minotech Biotechnology
Agilent Technologies
SibEnzyme Ltd.
Nippon Gene Co., Ltd.
Takara Bio Inc.
Roche Applied Science
New England Biolabs
Toyobo Biochemicals
Molecular Biology Resources - CHIMERx
Promega Corporation
Sigma Chemical Corporation
EURx Ltd.
SinaClon BioScience Co.
Minimum size of restriction enzyme binding site:
Fasta input:
Color scoring should ignore one off-target without mismatches.
Displayed flanking sequence length in detailed view:
sgRNA length without PAM:
PAM-3':
NGG
NAG
NGA
NRG (R = A or G)
NNAGAAW (W = A or T)
NNNNGMTT (M = A or C)
NNGRRT (R = A or G)
Custom PAM:
Method for determining off-targets in the genome:
Off-targets with up to
mismatches in protospacer
(Hsu et al., 2013)
Off-targets may have no more than
mismatches in the protospacer seed region
(Cong et al., 2013)
Efficiency score:
Doench et al. 2014 - only for NGG PAM
Doench et al. 2016 - only for NGG PAM
Chari et al. 2015 - only NGG and NNAGAAW PAM's in hg19 and mm10
Xu et al. 2015 - only for NGG PAM, but can be used with other PAMs
Moreno-Mateos et al. 2015 - only for NGG PAM
G20
Repair profile prediction (Shen et al. 2018):
mESC (recommended when you don't know which cell type)
U2OS
HEK293
HCT116
K562
Don't calculate (saves time)
5' requirements for sgRNA:
GN or NG
GG
No requirements
Self-complementarity (Thyme et al.):
Check for self-complementarity
I intend to replace the leading nucleotides with "GG"
Check for complementarity versus backbone:
Standard backbone (AGGCTAGTCCGT)
Extended backbone (AGGCTAGTCCGT,ATGCTGGAA)
Custom backbone:
sgRNA length without PAM:
PAM-3':
NGG
NAG
NGA
NRG (R = A or G)
NNAGAAW (W = A or T)
NNNNGMTT (M = A or C)
NNGRRT (R = A or G)
Custom PAM:
Method for determining off-targets in the genome:
Off-targets with up to
mismatches in protospacer
(Hsu et al., 2013)
Off-targets may have no more than
mismatches in the protospacer seed region
(Cong et al., 2013)
Efficiency score:
Doench et al. 2014 - only for NGG PAM
Doench et al. 2016 - only for NGG PAM
Chari et al. 2015 - only for NGG and NNAGAAW PAM's in hg19 and mm10
Xu et al. 2015 - only for NGG PAM, but can be used with other PAMs
Moreno-Mateos et al. 2015 - only for NGG PAM
G20
5' requirements for sgRNA:
GN or NG
GG
No requirements
Self-complementarity (Thyme et al.):
Check for self-complementarity
I intend to replace the leading nucleotides with "GG"
Check for complementarity versus backbone:
Standard backbone (AGGCTAGTCCGT)
Extended backbone (AGGCTAGTCCGT,ATGCTGGAA)
Custom backbone:
Distance between guides:
to:
Max distance between offtargets:
sgRNA length without PAM:
PFS:
5' end of target (like in Cpf1) e.g. Cas13a:
3' end of target (like in Cas9):
sgRNA length without PAM:
5'-PAM:
TTN
TTTN
TTCN (CasX)
Non-standard:
Efficiency score:
Kim et al. 2018
None (will display zeros in "Efficiency" column)
Self-complementarity (Thyme et al.):
Check for self-complementarity
Check for complementarity versus backbone:
Standard backbone (AGGCTAGTCCGT)
Extended backbone (AGGCTAGTCCGT,ATGCTGGAA)
Custom backbone:
Distance between TALENs:
to:
Size of TALENs:
Number of mismatches to search for when finding off-targets:
0
1
2
G repeat variable diresidue (RVD):
NN
NH
Design primers
Product size:
From:
to:
Primer size:
From:
to:
Optimal:
Primer Tm:
From:
to:
Optimal:
Minimum distance from primer to target site:
Paste Target
Options
Reset Options
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